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一.產品名稱：Recombinant Human β-NGF/重組蛋白β-NGF

二.Catalog Number:AF-450-01

三.Synonyms:NGF-β

四.Description:

β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. The functional form of human β-NGF is a noncovalently disulfide-linked homodimer, of two 13.5 kDa polypeptide monomers (238 total amino acid residues). The three disulfide bonds are required for biological activity. Manufactured using all non-animal reagents.

五.AA Sequence:

SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVR

六.Source:E.coli

七.Purity:Greater than 98% by SDS-PAGE gel and HPLC analyses.

八.Biological Activity:

Assay 1:Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The expected ED₅₀ is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁶ units/mg.
Assay 2:Determined by its ability to stimulate chick E9 DRG neurite outgrowth. The expected ED₅₀ is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁶ units/mg.

九.Cross Reactivity:Chicken, Hamster, Mouse, Rat, Human